Question
You try to biochemically test if your candidate FUD gene encodes a Gort receptor, but these
experiments do not show a clear result. A friend who is
an expert on chemical synthesis makes you +(+R) and -
(-S) structural isomers of Gort. The structures are show
+R'I-GORT
-(51-GORT
here;
You apply each of these isomers to wild type Klaatu barada niko cells and see the following result
in the presence of each Gort isomer. Mock is where no Gort Mock
+(R)-GORT -(5)-GORT
compound was added. From this information come up with a
genetic screen to identify mutations in the Gort receptor. Briefly
explain your logic (3 marks).
T
Answer
My logic would be to use a genetic screen to identify mutations in the Gort receptor by comparing the response of the wild type Klaatu barada niko cells to the +(+R) and -(-S) structural isomers of Gort. If the response to the +(+R) isomer is significantly different from the response to the -(-S) isomer, then this suggests that the FUD gene encodes a Gort receptor. If the response to both isomers is similar, then this suggests that the FUD gene does not encode a Gort receptor.